CCA 3D Sample

We discuss the development of MacConkey’s agar and Defined Substrate Technology (DST) as methods for coliform detection in the 1890s. Additionally, it explains how the use of antibiotic supplements can increase specificity in nutrient-dense environments like agar, and how the enzyme-based reaction of x-gluc and beta-glucuronidase can help detect the presence of E. coli in CCA plates.